Title
Energetic electron assisted synthesis of highly tunable temperature-responsive collagen/elastin gels for cyclic actuation: macroscopic switching and molecular origins
Author
Nils Wilharm, Tony Fischer, Florian Ott, Robert Konieczny, Mareike Zink, Annette G. Beck-Sickinger, Stefan G. Mayr
Year
2019
Journal
Scientific Reports
Abstract
Thermoresponsive bio-only gels that yield sufficiently large strokes reversibly and without large hysteresis at a well-defined temperature in the physiological range, promise to be of value in biomedical application. Within the present work we demonstrate that electron beam modification of a blend of natural collagen and elastin gels is a route to achieve this goal, viz. to synthesize a bioresorbable gel with largely reversible volume contractions as large as 90% upon traversing a transition temperature that can be preadjusted between 36 °C and 43 °C by the applied electron dose. Employing circular dichroism and temperature depending confocal laser scanning microscopy measurements, we furthermore unravel the mechanisms underlying this macroscopic behavior on a molecular and network level, respectively and suggest a stringent picture to account for the experimental observations.
Full Article
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Thermal stability, Chemical stability, Biochemistry