Engineered Protein Polymer-Gold Nanoparticle Hybrid Materials for Small Molecule Delivery

By Min Dal, JA Frezzo, E Sharma, R Chen, N Singh, C Yuvienco, E Caglar, S Xiao,2 A Saxena, JK Montclare

July 28, 2017

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Title

Engineered Protein Polymer-Gold Nanoparticle Hybrid Materials for Small Molecule Delivery

Author

Min Dal, JA Frezzo, E Sharma, R Chen, N Singh, C Yuvienco, E Caglar, S Xiao,2 A Saxena, JK Montclare

Year

2016

Journal

Journal of Nanomedicine and Nanotechnology

Abstract

We have fabricated protein polymer-gold nanoparticle (P-GNP) nanocomposites that exhibit enhanced binding and delivery properties of the small hydrophobic molecule drug, curcumin, to the model breast cancer cell line, MCF-7. These hybrid biomaterials are constructed via in situ GNP templated-synthesis with genetically engineered histidine tags. The P-GNP nanocomposites exhibit enhanced small molecule loading, sustained release and increased uptake by MCF-7 cells. When compared to the proteins polymers alone, the P-GNPs demonstrate a greater than 7-fold increase in curcumin binding, a nearly 50% slower release profile and more than 2-fold increase in cellular uptake of curcumin. These results suggest that P-GNP nanocomposites serve as promising candidates for drug delivery vehicles.

Full Article

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828936/

Instrument

J-815

Keywords

Circular dichroism, Nanostructures, Polymers, Secondary structure, Thermal stability, Pharmaceutical, Biochemistry, Materials