Title
Extending the range of supercritical fluid chromatography by use of water-rich modifiers
Author
Jinchu Liu, Erik L. Regalado, Ingrid Mergelsberg, Christopher J. Welch
Year
2013
Journal
Organic and Biomolecular Chemistry
Abstract
In this study we investigate the recently reported use of water-containing modifiers for separation and purification of hydrophilic compounds by supercritical fluid chromatography. Improved peak shape is obtained for a variety of glycosides and otherwise hydrophilic compounds when 5% water is added to the methanol co-solvent used in SFC separations, and examples of the use of this approach in preparative SFC purifications are presented.
Instrument
J-810
Keywords
Circular dichroism, Stereochemistry