Title
Fabrication of thermoresponsive near-infrared fluorescent gold nanocomposites and their thermal manipulation
Author
Nobuo Uehara, Yoshikuni Numanami
Year
2017
Journal
Sensors and Actuators B: Chemical
Abstract
Thermoresponsive gold nanocomposites emitting near-infrared fluorescence were fabricated and used for fluorometric staining of HeLa cells. The gold nanocomposites were composed of two types of gold nanoparticles conjugated with a thermoresponsive polymer bearing triethylenetetramine groups. One type of gold nanoparticle was fluorescent and had a particle size of 2 nm, and the other type was non-fluorescent, had a particle size of 6 nm, and exhibited an absorbance band at 310 nm. Conjugation with the thermoresponsive polymers brought the two types of gold nanoparticle together, forming gold nanocomposites that fluoresced at 830 nm. Changing the solution temperature induced the phase transition of the thermoresponsive polymers in the gold nanocomposites, altering the fluorescence of the gold nanocomposites. The gold nanocomposites accumulated in HeLa cells after 24 h incubation and were detected fluorometrically.
Instrument
FP-6300
Keywords
Fluorescence, Nanostructures, Polymers, Thermal stability, Inorganic chemistry, Materials, Sensors