Title
Flexible amine-functionalized triphenylamine derivative as a fluorescent “light-up” probe for G-quadruplex DNA
Author
Ming-Qi Wang, Shuo Liu, Cai-Ping Tang, Aun Raza, Shuo Li, Lie-Xiong Gao, Jing Sun, Song-Ping Guo
Year
2017
Journal
Dyes and Pigments
Abstract
In this study, a flexible amine-functionalized triphenylamine dye 2 was developed and utilized as a G4 DNA fluorescent probe. The selectivity, sensitivity and binding properties of the dye 2 interacting with G4 DNAs were investigated. The dye 2 illustrated in the fluorescence titration assays were preferentially bound with G4 DNAs with significant “light-up” effect. The detection limit value of 2 for the G4 DNA was below 10 nM in solution. CD spectroscopy analysis revealed that 2 could promote the G-rich sequence to adapt an antiparallel G4 structure. These results indicated the dye 2 showed stronger binding affinity and fluorescence response to G4 DNA than our previous reported triphenylamine-quinolinium scaffold (1b) due to the incorporation of a flexible amine moiety. Therefore, this study gave certain crucial factors on developing of effective fluorescence probes for G4 DNA. The cellular application of 2 in cancer cells HepG2 was also demonstrated.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Materials, Biochemistry