Title
Functionalization of Fe3O4@SiO2 magnetic nanoparticles with nicotinamide and in vitro DNA interaction
Author
Nahid Shahabadi, Alireza akbari, Mina Jamshidbeigi, Monireh Falsafi
Year
2016
Journal
Journal of Molecular Liquids
Abstract
In the present work, the synthesis, characterization and DNA binding study of water-soluble γ-Fe2O3@SiO2-nicotinamide magnetic nanoparticles (MNPs) were undertaken. The Fe3O4 nanoparticles were synthesized via chemical co-precipitation and coated by silica according to sol-gel method. Then, Fe3O4@SiO2 MNPs were functionalized with an antioxidant drug, namely “nicotinamide”, to obtain new water-soluble magnetic nanoparticles for biological applications. The MNPs were subsequently characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and transmission electron microscopy (TEM) analyses. According to the XRD and FT-IR data, the iron oxide NPs were identified to be in magnetite phase (Fe3O4). The FT-IR analysis showed that the MNPs were successfully coated with SiO2 and nicotinamide drug. The morphology of γ-Fe2O3@SiO2-nicotinamide MNPs was recognized to be spherical in TEM images where they were observed to have a uniform size distribution with an average diameter of 28 nm. Finally, biological applications of the γ-Fe2O3@SiO2-nicotinamide MNPs were investigated in vitro (DNA-binding study). The interactions between the γ-Fe2O3@SiO2-nicotinamide MNPs with calf-thymus DNA (ct-DNA) were studied by UV–vis, fluorescence and CD spectroscopy. The results showed that DNA aggregated on the γ-Fe2O3@SiO2-nicotinamide MNPs via groove binding.
Instrument
FP-6200
Keywords
Fluorescence, Ligand binding, DNA structure, Nanostructures, Materials, Biochemistry, Medicinal