Title
G-quadruplex DNA fluorescence sensing by a bis-amine-substituted styrylquinolinium dye
Author
Ming-Qi Wang, Yuan Wu, Zi-Yu Wang, Qiu-Yun Chen, Fu-Yan Xiao, Yu-Chi Jiang, Amy Sang
Year
2017
Journal
Dyes and Pigments
Abstract
Searching for specific G-quadruplex DNA probes is important for study of the function of G-rich gene sequence, as well as design of novel effective anticancer drugs. In this paper, a novel bis-amine-substituted styrylquinolinium dye (BSAQ) was designed and synthesized to enhance the performance for the application as a G-quadruplex DNA probe. The studies on BSAQ with different DNA forms showed that it could be used as a colorimetric and red-emitting fluorescent probe for G-quadruplex DNA. The limits of detection of BASQ with various G-quadruplex DNAs were found to below 1 nM. CD spectroscopy analysis revealed that BSAQ did not induce the G-rich sequence folding into G-quadruplex structure. These results of this study gave some crucial factors on developing of effective probes for G-quadrupex DNA applications.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Ligand binding, Biochemistry, Materials