Title
Hybrid Self-Assembling Peptide/Gelatin Methacrylate (GelMA) Bioink Blend for Improved Bioprintability and Primary Myoblast Response
Author
Mitchell Boyd-Moss, Kate Firipis, Anita Quigley, Aaqil Rifai, Artur Cichochki, Sarah Whitty,Catherine Ngan, Chaitali Dekiwadia, Benjamin Long, David R. Nisbeto, Robert Kapsa, Richard Williams
Year
2021
Journal
ADVANCED NANOBIOMED RESEARCH
Abstract
Organ fabrication as the solution to renewable donor demands requires the ability to spatially deposit viable cells into biologically relevant constructs; necessitating reliable and effective cell deposition through bioprinting and the subsequent ability to mature. However, effective bioink development demands advances in both printability and control of cellular response. Effective bioinks are designed to retain shape fidelity, influence cellular behavior, having bioactive morphologies stiffness and highly hydrated environment. Hybrid hydrogels are promising candidates as they reduce the need to re-engineer materials for tissue-specific properties, with each component offering beneficial properties. Herein, a multicomponent bioink is developed whereby gelatin methacrylate (GelMA) and fluorenylmethoxycarbonyprotected self-assembling peptides (Fmoc-SAPs) undergo coassembly to yield a tuneable bioink. This study shows that the reported fibronectin-inspired fmoc-SAPs present cell attachment epitopes RGD and PHSRN in the form of bioactive nanofibers; and that the GelMA enables superior printability, stability in media, and controlled mechanical properties. Importantly, when in the hybrid format, no disruption to either the methacrylate crosslinking of GelMA, or self-assembled peptide fibril formation is observed. Finally, studies with primary myoblasts show over 98% viability at 72 h and differentiation into fused myotubes at one and two weeks demonstrate the utility of the material as a functional bioink for muscle engineering.
Instrument
J-815
Keywords
biologically, GelMA,