Title
Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft
Author
Lijun Xing, Yifeng Xu, Keyong Sun, Hong Wang, Fengguo Zhang, Zhengpin Zhou, Juan Zhang, Fang Zhang, Bilgen Caliskan, Zheng Qiu, Min Wang
Year
2018
Journal
Scientific Reports
Abstract
The expression level of folate receptor alpha (FRα) is located highly rate in ovarian cancer though it is remained absent in normal tissues. This highly tumor restricted expression profile makes FRα a promising target for tumor therapy and diagnosis. In this research we report a FRα binding peptide C7(Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser) discovered by phage display and this peptide showed specific binding to FRα expressing cells by cell ELISA and flow cytometry. Tumor targeting ability of C7 was proved in vivo by both phage homing experiment and fluorescence imaging. C7 can be internalized by SKOV3 cells and its affinity to FRα was determined by MST. The molecular recognition was revealed by structure modeling, suggesting its binding mode with FRα.
Full Article
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Biochemistry