Title
Identification of acetylated diether lipids in halophilic Archaea
Author
Cosimo Kropp, Julius Lipp, Anna Lena Schmidt, Christina Seisenberger, Mona Linde, Kai-Uwe Hinrichs, Patrick Babinger
Year
2022
Journal
MicrobiologyOpen
Abstract
Archaea-type ether lipids have been found recently in Bacillales. They are monoether lipids with a C35 isoprenoid chain and are diacetylated by the O-acetyltransferase YvoF. Phylogenetic studies implied that YvoF homologs also occur in Halobacteria (Archaea). Here we show that YvoF from Haloferax volcanii has ether-lipid-specific acetyltransferase activity in vitro. Furthermore, we provide a mass spectrometric identification of acetylated archaeol and acetylated archaetidylglycerol in H. volcanii and Halobacterium salinarum for the first time.
Instrument
V-670
Keywords
lipids, C35, Archaea