Title
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1–40 peptide and silk fibroin thin films
Author
J. M. Goncalves, L.R. Lima, M.L. Moraes, S.J.L. Ribeiro
Year
2016
Journal
Materials Science and Engineering: C
Abstract
Layer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV–vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL− 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Materials, Biochemistry