Title
Increasing the Assembly Efficacy of Peptidic β-Sheets for a Highly-Sensitive HIV Detection
Author
Hongyan Cao, Ye Liu, Hongyan Sun, Zulan Li, Yuan Gao, Xinli Deng, Yiming Shao, Yulong Cong, Xingyu Jiang
Year
2020
Journal
Analytical Chemistry
Abstract
Our recent publication illustrates the critical role of phenylalanine-mediated aromatic-aromatic interactions in determining the assembly of peptidic β-sheets. However, the effect of phenylalanine number on regulating the assembly efficacy of peptidic β-sheets remains poorly understood. We herein evaluate the assembly efficacy of β-sheets of a series of oligo-peptides which contain 0, 1, 2 or 3 phenylalanine in their molecular backbones. In our assembly system, two phenylalanine (2F) is the minimum number for driving the assembly of β-sheets of oligo-peptides. Oligo-peptides with three phenylalanine (3F) show significantly increased assembly efficacy of β-sheets than that with 2F. These results suggest a positive correlation between phenylalanine number and assembly efficacy of β-sheets. By improving the assembly efficacy of β-sheets, we further develop a highly-sensitive HIV analytical system in which the specific-binding of β-sheets with Congo Red induces enhanced fluorescence. For HIV p24 detection, 3F-based analytical system (0.61 pg/ml) shows a significantly lower limit of detection (LOD) than 2F-based analytical system (2.44 pg/ml), both of which are more sensitive than commercial ELISA (5 pg/ml) used in the clinic. This work not only illustrates the effect of phenylalanine number on regulating the assembly efficacy of β-sheets, but also provides a guideline for the construction of a highly-sensitive analytical system of disease diagnosis.
Instrument
J-1500
Keywords
Circular dichroism, Secondary structure, Chemical stability, Biochemistry