Title
Induced Circularly Polarized Luminescence for Revealing DNA Binding with Fluorescent Dyes
Author
Marcin Górecki, Francesco Zinna, Tarita Biver, Lorenzo Di Bari
Year
2017
Journal
Journal of Pharmaceutical and Biomedical Analysis
Abstract
To the best of our knowledge this is the first report on the application of induced circularly polarized luminescence (CPL) for sensing the binding of fluorescent dyes to double stranded DNA. Using Thiazole Orange (TO) and 4′,6-diamidino-2-phenylindole (DAPI) as models, we show utility and limitations of CPL in DNA binding studies. The results obtained indicate that CPL can be used as a new chiroptical tool for this purpose, however, special attention while recording CPL data must be used, in order to exclude measurement artefacts caused by linear polarization components.
Instrument
J-715
Keywords
Circular dichroism, Induced CD, DNA structure, Ligand binding, Biochemistry