Inhibition of Alzheimer Amyloid Aggregation with Sulfated Glycopolymers

By Yoshiko Miura, Kikuko Yasuda, Kiyofumi Yamamoto, Mihoko Koike, Yoshihiro Nishida, Kazukiyo Kobayashi

May 22, 2018

  • Year

  • Technique

  • Industry

Title

Inhibition of Alzheimer Amyloid Aggregation with Sulfated Glycopolymers

Author

Yoshiko Miura, Kikuko Yasuda, Kiyofumi Yamamoto, Mihoko Koike, Yoshihiro Nishida, Kazukiyo Kobayashi

Year

2007

Journal

Biomacromolecules

Abstract

Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid β peptides (Aβ(1−42), Aβ(1−40), and Aβ(25−35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid β peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the β-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect.

Full Article

http://pubs.acs.org/doi/abs/10.1021/bm0701402

Instrument

FP-6500

Keywords

Fluorescence, Polymers, Aggregation, Biochemistry