Title
Inhibition of HSA Fibrillation by Two Dimensional Nanoparticles
Author
Sudipta Bag, Rishav Mitra, Sunando DasGupta, Swagata Dasgupta
Year
2017
Journal
The Journal of Physical Chemistry B
Abstract
The formation and deposition of amyloid fibrils have been linked to the pathogenesis of numerous debilitating neurodegenerative disorders. Serum albumins serve as good model proteins for understanding the molecular mechanisms of protein aggregation and fibril formation. Graphene based nanotherapeutics appear to be promising candidates for designing inhibitors of protein fibrillation. The inhibitory effect of graphene oxide (GO) nanoparticles on the fibrillation of human serum albumin (HSA) in an in vitro mixed solvent system has been investigated. The methods used include ThT fluorescence, ANS binding, Trp fluorescence, circular dichroism, fluorescence microscopy, FESEM and HRTEM. It was observed that GO inhibits HSA fibrillation and forms agglomerates with β-sheet rich pre-fibrillar species. Binding of GO prevents the formation of mature fibrils with characteristic cross-β sheet but does not promote refolding to the native state.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Ligand binding, Aggregation, Nanostructures, Materials, Biochemistry