Title
Investigations of Anti-Inflammatory Activity of a Peptide-Based Hydrogel using Rat Air Pouch Model
Author
Pramod Kumar Gavel, Hamendra Singh Parmar, Versha Tripathi, Narendra Kumar, Ankan Biswas, Apurba K Das
Year
2018
Journal
ACS Applied Materials & Interfaces
Abstract
The growing area of biomaterial sciences has drawn a broad attention over recent years on the development of peptide-based biocompatible materials with inherent therapeutic potentials. Here, we developed Amoc (9-anthracenemethoxycarbonyl)-capped dipeptide-based biocompatible, injectable, thixotropic and self-healable hydrogel. In vitro cytotoxicity of the hydrogel was investigated with human embryonic kidney cell (HEK293) line. We observed that synthesized peptide is non-cytotoxic. The hydrogel showed an antibacterial efficacy against Gram-positive and Gram-negative bacteria. In vivo anti-inflammatory activity of the hydrogel was investigated using the rat air pouch model of acute inflammation. The major parameters considered for the anti-inflammatory study were exudate volume, total and differential white blood cells count, tissue histology and lipid peroxidation assay. These experimental data suggest biocompatibility and potential therapeutic applications of peptide hydrogel in inflammation.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Materials, Chemical stability, Biochemistry