Iridium Complexes as a Roadblock for DNA Polymerase during Amplification

By Falguni Chandra, Prashant Kumar, Suman Kumar Tripathi, Srikanta Patra, Apurba L. Koner

July 28, 2017

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Title

Iridium Complexes as a Roadblock for DNA Polymerase during Amplification

Author

Falguni Chandra, Prashant Kumar, Suman Kumar Tripathi, Srikanta Patra, Apurba L. Koner

Year

2016

Journal

ChemMedChem

Abstract

Iridium-based metal complexes containing polypyridyl-pyrazine ligands show properties of DNA intercalation. They serve as roadblocks to DNA polymerase activity, thereby inhibiting the polymerization process. Upon the addition of increasing concentrations of these iridium complexes, a rapid polymerase chain reaction (PCR)-based assay reveals the selective inhibition of the DNA polymerization process. This label-free approach to study the inhibition of fundamental cellular processes via physical roadblock can offer an alternative route toward cancer therapy.

Full Article

http://onlinelibrary.wiley.com/wol1/doi/10.1002/cmdc.201600101/abstract

Instrument

J-815

Keywords

Circular dichroism, Inorganic chemistry, DNA structure, Pharmaceutical, Biochemistry