Title
Logic Catalytic Interconversion of G-Molecular Hydrogel
Author
Ruibo Zhong, Mingshu Xiao, Changfeng Zhu, Xizhong Shen, Qian Tang, Weijia Zhang, Lihua Wang, Shiping Song, Xiangmeng Qu, Hao Pei, Cheng Wang, Li Li
Year
2018
Journal
ACS Applied Materials & Interfaces
Abstract
By incorporating hemin into G-quadruplex (G4) during cation-templated self-assembly between guanosine and KB(OH)4, we have constructed an artificial enzyme hydrogel (AEH)-based system for the highly sensitive and selective detection of Pb2+. The sensing strategy is based on a Pb2+-induced decrease in AEH activity. Because of the higher efficiency of Pb2+ for stabilizing G4compared with K+, the Pb2+ ions substitute K+ and trigger hemin release from G4, thus giving rise to a conformational interconversion accompanied by the loss of enzyme activity. The Pb2+-induced catalytic interconversion endows the AEH-based system with high sensitivity and selectivity for detecting Pb2+. As a result, the AEH-based system shows an excellent response for Pb2+ in the range from 1 pM to 50 nM with a limit of detection of ∼0.32 pM, which is much lower than that of the previously reported G4-DNAzyme. We also demonstrate that this AEH-based system exhibits high selectivity toward Pb2+ over other metal ions. Furthermore, two two-input INHIBIT logic gates have been constructed via switching of the catalytic interconversion induced by K+ and Pb2+ or K+and pH. Given its versatility, this AEH-based system provides a novel platform for sensing and biomolecular computation.
Full Article
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Materials, Biochemistry