Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules

By Marco Caterino, Ariel A. Petruk, Alessandro Vergara, Giarita Ferraro, Daniela Marasco, Fabio Doctorovich, Dario A. Estrin, Antonello Merlino

July 28, 2017

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Title

Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules

Author

Marco Caterino, Ariel A. Petruk, Alessandro Vergara, Giarita Ferraro, Daniela Marasco, Fabio Doctorovich, Dario A. Estrin, Antonello Merlino

Year

2016

Journal

Dalton Transactions

Abstract

A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties.

Full Article

http://pubs.rsc.org/en/content/articlelanding/2016/dt/c6dt01685e

Instrument

J-710

Keywords

Circular dichroism, Secondary structure, Protein denaturation, Ligand binding, Biochemistry