Title
Measuring fluorescence polarization with a dichrometer
Author
John C. Sutherland
Year
2017
Journal
Analytical Biochemistry
Abstract
A method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.
Instrument
J-1500
Keywords
Circular dichroism, Linear dichroism, Anisotropy, Fluorescence