Title
Microbial transformation of glycyrrhetinic acid and potent neural anti-inflammatory activity of the metabolites
Author
Yuan Ma, Ji-Mei Liu, Ri-Dao Chen, Xi-Qiang An, Jun-Gui Dai
Year
2017
Journal
Chinese Chemical Letters
Abstract
The microbial transformation of glycyrrhetinic acid (1) by Cunninghamella blakesleana CGMCC 3.970 led to the production of five new metabolites (2–6). The structures of the metabolites were determined by extensive spectroscopic (HR-ESIMS, 1D and 2D NMR) data analyses. The involved reactions exhibited specific hydroxylations at C-24, C-7, and C-15, and oxidation at C-3. Moreover, compounds 2, 5, and 6 showed significant neural anti-inflammatory activity by inhibiting lipopolysaccharide-induced NO production in mouse microglia BV2 cells with IC50 values of 0.76, 0.94, and 0.16 μmol/L, respectively
Instrument
J-815
Keywords
Circular dichroism, Absolute configuration, Natural products, Medicinal