Title
Molecular Determinant Modulates Thermal Recovery Kinetics and Structural Integrity of the Bacterial BLUF Photoreceptor
Author
Meenakshi Tanwar, Smita Nahar, Sahil Gulati, Sindhu Kandoth Veetil, Suneel Kateriya
Year
2016
Journal
FEBS Letters
Abstract
BLUF domains are flavin-based photoreceptors which receive the blue light signal and are involved in the sensory transduction. We report a short BLUF photoreceptor (SnfB) in Stenotrophomonas sp. We have investigated photodynamic properties of C-terminus truncated and several mutated SnfB proteins. Deletion of the extended C-terminus residues alters the thermal recovery kinetics and also affects the integrity of the SnfB protein. Mutagenesis studies demonstrated that the conserved residues within and outside the flavin binding pocket also regulates the photocycle properties of the protein. These studies suggest that the C-terminus residues outside the BLUF domain can tune the photodynamic properties of the BLUF protein.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Biochemistry