Molecular recognition of nucleic acids: Coralyne binds strongly to poly(A)

By Feifei Xing, Guangtao Song, Jinsong Ren, Jonathan B. Chaires, Xiaogang Qu

July 28, 2017

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Title

Molecular recognition of nucleic acids: Coralyne binds strongly to poly(A)

Author

Feifei Xing, Guangtao Song, Jinsong Ren, Jonathan B. Chaires, Xiaogang Qu

Year

2005

Journal

FEBS Letters

Abstract

The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant (K a) of (1.8 ± 0.3) × 106 M−1. Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 °C, for the conditions of our study.

Full Article

http://onlinelibrary.wiley.com/wol1/doi/10.1016/j.febslet.2005.07.091/abstract

Instrument

J-810

Keywords

Circular dichroism, Ligand binding, Thermodynamics, Thermal stability, Biochemistry