Multivariate curve resolution applied to the simultaneous analysis of electrochemical and spectroscopic data: Study of the Cd(II)/glutathione-fragment system by voltammetry and circular dichroism spectroscopy
Aristides Alberich, Cristina Ariño, José Manuel Díaz-Cruz, Miquel Esteban
Analytica Chimica Acta
Multivariate curve resolution with alternating least squares (MCR-ALS) is applied for the first time to the simultaneous analysis of electrochemical and spectroscopic data. Then, a data analysis is done with augmented matrices constituted by Differential Pulse Polarography and Circular Dichroism data submatrices. The use of proper, and different for each submatrix, constrains in the iterative ALS optimization allows to obtain chemically meaningful results constituted by a common matrix containing the concentration profiles, and two matrices with the pure electrochemical and spectroscopic signals. MCR-ALS is applied to the study of the complexation of Cd by Cys-Gly, a glutathione-fragment of great interest for understanding metal-phytochelatins complexation.
Circular dichroism, Chemometrics