New urushiols with platelet aggregation inhibitory activities from resin of Toxicodendron vernicifluum
Ya Xie, Jie Zhanga, Wenyuan Liu, Ning Xie, Feng Feng, Wei Qu
Eight new urushiol-type compounds (1–7b), along with seven known compounds were isolated from the resin of Toxicodendron vernicifluum Stokes. Their structures were determined by extensive spectroscopic methods, included 1H NMR, 13C NMR, HMQC, HMBC, HRESIMS, EI-MS in combination with CD methods. All the compounds except 7aand 7b were evaluated for their anti-platelet aggregation activities in vitro. Among them, compound 5 (IC50 = 5.12 ± 0.85 μmol/L), with a vic-diol moiety in the long alkyl chain showed the most potent inhibitory of platelet aggregation activity induced by ADP. In addition, compound 6 showed the effect of anti-platelet aggregation induced by AA with the IC50 value of 3.09 ± 0.70 μmol/L. Thus, these compounds might be the active components to the traditional use of Resina Toxicodendri for breaking up blood stasis, which could be related to the anti-platelet aggregation.
Circular dichroism, Absolute configuration, Pharmaceutical