Title
Novel Silica-Forming Peptides Derived from Ectocarpus Siliculosus
Author
Ki Baek Yeo, Mi Ran Ki, Ki Sung Park, Seung Pil Pack
Year
2017
Journal
Process Biochemistry
Abstract
A synthetic R5 peptide showed the silica precipitation activity when added to silicic acid solution under ambient conditions. However, R5 peptide showed no silica precipitation activity below pH 7, an important pH range for biological applications. Here, we reported new silica-forming peptides (SFP), named EctP1 and EctP2. They showed better silica deposition ability than the R5 peptide. Particularly, at pH 6, EctP1 showed silica deposition activity while R5 did not. EctP1, EctP2, and R5 were genetically fused to the C-terminus of green fluorescent protein (GFP). GFP-SFP fusion proteins showed silicification ability, and GFP-EctP1 fusion protein showed the capacious silicification activity at wide pH range. The GFP-EctP2 fusion proteins showed higher protein expression levels than other fusion proteins. Furthermore, silicified GFP-SFP fusion protein exhibited an organic-inorganic complex form. These results indicate that the SFP fusion system is a novel tool for immobilizing biomolecules on silica material for biological and industrial applications.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Ligand binding, Biochemistry