Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy

September 22, 2019

Title

Observation of Molecular Changes of a Necrotic Tissue from a Murine Carcinoma by Fourier-Transform Infrared Microspectroscopy

Author

Tetsushi Yamada, Norio Miyoshi, Toru Ogawa, Kenichi Akao, Masaru Fukuda, Toshiyuki Ogasawara, Yoshimasa Kitagawa and Kazuo Sano

Year

2002

Journal

Clinical Cancer Research

Abstract

Purpose: Our purpose is to develop infrared (IR) microspectroscopy as a new optical diagnostic tool to support conventional lightscopic techniques in investigating the viability of carcinoma tissues and to develop its use in the evaluation of the early effects of anticancer therapy by
monitoring the IR spectra in the necrotic area.

Experimental Design: We evaluated the tissue which amassed for 4 weeks after the isotransplantation of mouse squamous cell carcinoma into the thigh of mice. The borders of the necrotic area of frozen tissue specimens were investigated by Fourier-transform IR microspectroscopy and conventional histological staining.

Results: A significantly higher accumulation of cholesterol was observed in the necrotic tissue of a carcinoma. The mechanism of this phenomenon is hitherto unrecognized. We proposed that the accumulated cholesterol may lie extracellularly as a result of the ruptured plasma and internal membranes after the swelling of the necrotic cells brought on by hypoxia. The analysis of the secondary structure of protein revealed that the amounts of -sheet increased significantly in striking contrast to the decreasing amounts of -helix in a necrotic area of a carcinoma. It is plausible that this structural conversion of protein was because of lipidautooxidation products, such as cholesterol oxide but not cholesterol itself, which possesses cell toxicity and could be generated in a necrotic area.

Conclusions: We conclude that it will be possible to evaluate the efficacy of the clinical treatment of carcinoma by monitoring subtle biological changes of cholesterol absorbance in the early stage of necrosis because of anticancer treatment.

Instrument

FTIRM-410M/IRT-30M 

Keywords

FTIR microscopy, Murine Carcinoma, FTIR, secondary structure