Optimization, chain conformation and characterization of exopolysaccharide isolated from Leuconostoc mesenteroides DRP105
Hanwen Xing, Renpeng Du, Fangkun Zhao, Ye Han, Huazhi Xiao, Zhijiang Zhou
International Journal of Biological Macromolecules
Response surface methodology (RSM) was used to optimize the fermentation condition of exopolysaccharide (EPS) producing strain Leuconostoc mesenteroidesDRP105. Result showed that the optimum condition was sucrose 86.83 g/L, tryptone15.47 g/L, initial pH 7.18 and maximum yield was 53.79 ± 0.78 g/L in 36 h fermentation. Chain conformation was characterized by Congo red test, β-elimination and circular dichroism (CD), which indicated that the EPS was O-linkage and exhibited random coil structure in aqueous solution. CD results concluded hydrogen-bond interaction and chirality might be connected with concentration. Purified EPS has a higher degradation temperature of 279.42 °C, suggesting high thermal stability of the EPS. The absolute value of zeta potential and particle size were enhanced with increasing concentration. Crude EPS and its purified fraction were found to have moderate DPPH, hydroxyl, superoxide anion radicals scavenging activities and reducing power. This study provided a high yield EPS with unique characteristics for industrial applications.
Circular dichroism, Secondary structure, Biochemistry