Title
Plant Protein Amyloid Fibrils for Multifunctional Sustainable Materials
Author
Ting Li, Jiangtao Zhou, Mohammad Peydayesh, Yang Yao, Massimo Bagnani, Ines Kutzli, Zhengxing Chen, Li Wang, Raffaele Mezzenga
Year
2023
Journal
Advanced Sustainable Systems
Abstract
Artificial functional materials based on amyloid fibrils are proven to be a promising strategy toward functional materials. However, scaling-up applications present sustainability concerns, as animal proteins are the main sources for fabricating amyloid fibrils. Plant-protein-based amyloid fibrils, a more sustainable alternative to animal proteins, are attracting increasing interests as building blocks in functional materials. Herein, 11 different sources from a wide range of plants are evaluated, and a comprehensive analysis of seven species of plant proteins, including kidney bean, black bean, cowpea, mung bean, chickpea, lentil, and pumpkin seed, with an excellent ability to form fibrils, is presented. A universal strategy for a diversity of plant protein extraction and fibrillization is applied. Flexible fibrils with a persistence length of ≈100 nm and rigid fibrils of several micrometers are discovered in 7S/8S and 11S subunits dominated protein, respectively. Structural evolution toward the β-sheet content on these proteinaceous assemblies is characterized by thioflavin T (ThT) intensity, circular dichroism (CD) spectra, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectra, and typical wide angle X-ray scattering (WAXS) spectra. Finally, their multifunctional applications are further explored and proven that these sustainable protein amyloids demonstrate excellent performance in renewable and degradable bioplastics, and in water purification membranes for heavy metal removal.
Instrument
J-815
Keywords
artificial functional materials, amyloid, protein, structure