Title
Preparation and photo-induced activities of water-soluble amyloid β-C60 complexes
Author
Naoki Hasunumaa, Masahiro Kawakamia, Hirotsugu Hiramatsu, Takakazu Nakabayashi
Year
2018
Journal
RSC Advances
Abstract
We have shown that fullerene (C60) becomes soluble in water by mixing fullerene and amyloid β peptide (Aβ40) whose fibril structures are considered to be associated with Alzheimer's disease. The water-solubility of fullerene arises from the generation of a nanosized complex between fullerene and the monomer species of Aβ40 (Aβ40-C60). The prepared Aβ40-C60 exhibits photo-induced activity with visible light to induce the inhibition of Aβ40 fibrillation and the cytotoxicity for cultured HeLa cells. The observed photo-induced phenomena result from the generation of singlet oxygen viaphotoexcitation, inducing oxidative damage to Aβ40 and HeLa cells. The oxidized Aβ40 following photoexcitation of Aβ40-C60 was confirmed by mass spectrometry.
Instrument
FP-6500
Keywords
Fluorescence, Aggregation, Protein structure, Biochemistry