Title
Probing Tyrosine Nitration with a Small Tb(III)-Metallopeptide
Author
Sánchez-Fernández, Rosalía, Agustín Sánchez-Temprano, David Esteban-Gómez, and Elena Pazos
Year
2023
Journal
ChemBioChem
Abstract
Tyrosine nitration, a post-translational modification (PTM) that takes place under nitrosative stress conditions, occurs through a non-enzymatic peroxynitrite-mediated reaction. Although protein nitration has long been considered an irreversible PTM involved in nitrosative stress-associated diseases, it has also been suggested to be a regulatory mechanism of signal transduction. Therefore, the development of tools that help to understand this protein modification is of great interest. Herein, we explore a Tb(III)-chelating metallopeptide to monitor tyrosine nitration. The luminescence of this probe decreases significantly between its non-nitrated and nitrated states, and this reduction in the luminescence intensity is directly related to the degree of tyrosine nitration after treatment with peroxynitrite. Remarkably, the luminescence intensity changes after nitration are not affected in the presence of complex biological media, which makes it a promising tool for understanding this protein modification.
Instrument
V-750
Keywords
Nitrosative stress,protein tyrosine nitration,luminescent probes,lanthanides,metallopeptides