Title
Production of the Renewable Extremophile Lipase; Valuable Biocatalyst with Potential Usage in Food Industry
Author
Mina Memarpoor-Yazdi, Hamid Reza Karbalaei-Heidari, Khosro Khajeh
Year
2017
Journal
Food and Bioproducts Processing
Abstract
In the food industry, biocatalysts provide ecofriendly procedures to accomplish biochemical reactions with high specificity in moderate conditions. In this regard, enzymes are welcomed as process aids in food processing. Microbial lipases, especially of extremophiles, are one of the most useful enzymes for biotechnology and food industry. Accordingly, thermo-halophile GDSL lipase-encoding gene from Rhodothermus marinus, RD, was cloned and expressed in Escherichia coli. The enzyme revealed the highest hydrolytic activity)1055.3 Umg−1) towards p-nitrophenyl butyrate (C4) at 70 °C/pH 8.5 and retained 78.6% of its initial activity after 60 min incubation at this temperature. The enzyme was recovered by covalent immobilization on chitosan coated Fe3O4-nanoparticles (CS-MNPs) and the potential to synthesize aroma ester methyl acetate in n-hexane was investigated. The reaction yields were 22% and 67% for free and immobilized enzyme, respectively. This study provides a time-saving, economic, biocompatible and a renewable catalyst to synthesize natural flavor compounds. This biocatalyst has excellent potential in food industry and can be applied for lipid processing or to improve the aroma of foods. In addition to exhibiting good stability in high temperatures and organic solvents, RD lipase from Rhodothermus marinus can also be considered as an excellent candidate in organic synthesis in harsh industry conditions.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Tertiary structure, Chemical stability, Biochemistry, Food science