Reversible conformational changes in the parallel type G-quadruplex structure inside a thermoresponsive hydrogel

By Erika Hasuike, Aya Mizutani Akimoto, Reiko Kuroda, Ko Matsukawa, Yuki Hiruta, Hideko Kanazawa, Ryo Yoshida

July 28, 2017

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Title

Reversible conformational changes in the parallel type G-quadruplex structure inside a thermoresponsive hydrogel

Author

Erika Hasuike, Aya Mizutani Akimoto, Reiko Kuroda, Ko Matsukawa, Yuki Hiruta, Hideko Kanazawa, Ryo Yoshida

Year

2017

Journal

Chemical Communications

Abstract

The novel property of reversible regulation of parallel type G-quadruplexes with moderate temperature changes was discovered in thermoresponsive hydrogels using a universal chiroptical spectrophotometer 1 (UCS-1). This study is the first to report the regulation of the ssDNA steric structure by using a poly(N-isopropylacrylamide) (PNIPAAm) gel.

Full Article

http://pubs.rsc.org/en/content/articlelanding/2017/cc/c7cc00279c

Instrument

J-720

Keywords

Circular dichroism, DNA structure, Thermal stability, Materials, Biochemistry