Title
Role of nanoparticle size in self-assemble processes of collagen for tissue engineering application
Author
Mohan Vedhanayagam, Marimuthu Nidhin, Natarajan Duraipandy, Niranjan Dhanasekar Naresh, Ganesh Jaganathan, Mohan Ranganathan, Manikantan Syamala Kiran, Shoba Narayan, Balachandran Unni Nair, Kalarical Janardhanan Sreeram
Year
2017
Journal
International Journal of Biological Macromolecules
Abstract
Nanoparticle mediated extracellular matrix may offer new and improved biomaterial to wound healing and tissue engineering applications. However, influence of nanoparticle size in extracellular matrix is still unclear. In this work, we synthesized different size of silver nanoparticles (AgNPs) comprising of 10 nm, 35 nm and 55 nm using nutraceuticals (pectin) as reducing as well as stabilization agents through microwave irradiation method. Synthesized Ag-pectin nanoparticles were assimilated in the self-assemble process of collagen leading to fabricated collagen-Ag-pectin nanoparticle based scaffolds. Physico-chemical properties and biocompatibility of scaffolds were analyzed through FT-IR, SEM, DSC, mechanical strength analyzer, antibacterial activity and MTT assay. Our results suggested that 10 nm sized Ag-pectin nanoparticles significantly increased the denaturation temperature (57.83 °C) and mechanical strength (0.045 MPa) in comparison with native collagen (50.29 °C and 0.011 MPa). The in vitro biocompatibility assay reveals that, collagen-Ag-pectin nanoparticle based scaffold provided higher antibacterial activity against to Gram positive and Gram negative as well as enhanced cell viability toward keratinocytes. This work opens up a possibility of employing the pectin caged silver nanoparticles to develop collagen-based nanoconstructs for biomedical applications.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Nanostructures, Biochemistry, Materials