Title
Selective recognition of human telomeric G-quadruplex with designed peptide via hydrogen bonding followed by base stacking interactions
Author
Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy
Year
2019
Journal
RSC Advances
Abstract
We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.
Instrument
J-715, FP-8300
Keywords
Circular dichroism, Fluorescence, Ligand binding, G-quadruplex structure, DNA structure, Biochemistry