Title
Self-Emulsifying Phospholipid Preconcentrates for the Enhanced Photoprotection of Luteolin
Author
Yun-Shan Hsieh, Yih-Fung Chen, Yung-Yi Cheng, Wan-Yi Liu and Yu-Tse Wu
Year
2022
Journal
Pharmaceuticals
Abstract
"Exposure to ultraviolet B (UVB) leads to the overproduction of reactive oxygen species
(ROS), causing higher risks of skin disorders. Luteolin (Lut) is a naturally occurring antioxidant
that can absorb a broad range of ultraviolet light, but its water solubility and skin permeability are
limited and insufficient. The aim of the current study was to develop a Lut-loaded self-emulsifying
phospholipid preconcentrate (LSEPP) for enhancing the solubility, permeability, and photoprotective
activity of Lut. The designed formulations were firstly examined for their droplet size, zeta potential,
dispersity, and in vitro corneum permeability after dispensing the preconcentrate to form an emulsion;
the optimized formulation was further characterized for its emulsified morphology, compatibility
with excipients, stability in the preconcentrate form, and photoprotective activity by the HaCaT cell
model under the emulsified status. The optimized LSEPP formulation attained a smaller droplet size
(140.6 ± 24.2 nm) with the addition of 1,8-cineole and increased the permeability of Lut by 7-fold. As
evidenced in the cell model studies, the optimized LSEPP formulation can efficiently deliver Lut into
HaCaT cells after emulsification and result in a 115% better cell viability as well as a 203% stronger
ROS scavenging capability, compared with those of unformulated Lut after UVB irradiation. To sum
up, we have successfully developed an LSEPP formulation, which is a safe and promising topical
delivery system for enhancing the photoprotective effects of Lut."
Full Article
Instrument
FTIR-6800
Keywords
luteolin, self-emulsifying phospholipid preconcentrates, photoprotection