Title
Single Site Discrimination of Cytosine, 5‑Methylcytosine, and 5‑Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes
Author
Jean-Louis H. A. Duprey, Gemma A. Bullen, Zheng-yun Zhao, Dario M. Bassani, Anna F. A. Peacock, John Wilkie, James H. R. Tucker
Year
2016
Journal
ACS Chemical Biology
Abstract
The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.
Instrument
J-810
Keywords
Circular dichroism, Biochemistry