Solid phase synthesis and RNA-binding activity of an arginine-containing nucleopeptide

By G. N. Roviello, C. Vicidomini, S. Di Gaetano, D. Capasso, D. Musumeci, V. Roviello

July 28, 2017

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Title

Solid phase synthesis and RNA-binding activity of an arginine-containing nucleopeptide

Author

G. N. Roviello, C. Vicidomini, S. Di Gaetano, D. Capasso, D. Musumeci, V. Roviello

Year

2016

Journal

RSC Advances

Abstract

Here we report the solid phase synthesis and characterization (LC-ESIMS, CD) of a cationic nucleobase-containing α-peptide, composed of both L-arginine residues and L-lysine-based nucleoamino acids sequentially present in the structure. The binding properties of this novel basic nucleopeptide towards nucleic acids were investigated by CD spectroscopy which revealed the ability of the thymine-containing oligomer to bind both adenine-containing DNA (dA12) and RNA (poly rA) molecules inducing high conformational variations in the nucleic acid structures. Moreover, the artificial oligonucleotide inhibited the enzymatic activity of HIV reverse transcriptase, opening the door to the exploitation of novel antiviral strategies inspired to this molecular tool.

Full Article

http://pubs.rsc.org/en/content/articlelanding/2016/ra/c5ra25809j

Instrument

J-810

Keywords

Circular dichroism, Stereochemistry, Ligand binding, Biochemistry