Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR

July 28, 2017

Title

Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR

Author

Vinod Vikas Patil, Kwang-Hyun Park, Seung-Goo Lee, Euijeon Woo

Year

2016

Journal

Structure

Abstract

Positive phenol-degradative gene regulator (PoxR) is a σ54-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering.

Instrument

J-815

Keywords

Circular dichroism, Secondary structure, Biochemistry