Title
Structural elucidation and antioxidant activity of a novel Se-polysaccharide from Se-enriched Grifola frondosa
Author
Qian Li, Wei Wang, Yun Zhu, Yao Chen, Weijie Zhang, Ping Yu, Guanghua Mao, Ting Zhao, Weiwei Feng, Liuqing Yang, Xiangyang Wu
Year
2016
Journal
Carbohydrate Polymers
Abstract
Se-GFP-22, a heteropolysaccharide, with a weight-average Mw of 4.13 × 106 Da, was purified from the crude Se-polysaccharide (Se-GFP) isolated from fruit bodies of Se-enriched Grifola frondosa. Selenium was accumulated efficiently in Grifola frondosa during cultivation with Na2SeO3. The structure was investigated through FT-IR, GC, GC-MS, NMR, HPSEC-MALL-RI, particle size, Conge-red test, CD, AFM and SEM. Se-GFP-22 was deduced as a backbone chain of 1,4-α-D-Glcp units with a branched point at C6 of both 1,3,6-β-D-Manp and 1,4,6-α-D-Galp units. A typical absorption for selenium ester was existed in Se-GFP-22. Se-GFP-22 adopted as a spherical conformation with random coils. A novel Se-polysaccharide of different monosaccharide constituents, molecular weight, linkage types and high content of selenium has been isolated from G. frondosa. The antioxidant effect of Se-GFP-22 was more potent than that of G. frondosa polysaccharide (GFP-22), which may be influenced by the co-effect of polysaccharide and Se, molecular weight, degree of branching and configuration.
Instrument
J-815
Keywords
Circular dichroism, Polymers, Stereochemistry