Title
Structural interactions of a voltage sensor toxin with lipid membranes
Author
Mihaela Mihailescu, Dmitriy Krepkiy, Mirela Milescu, Klaus Gawrisch, Kenton J. Swartz, Stephen White
Year
2014
Journal
PNAS
Abstract
Protein toxins from tarantula venom alter the activity of diverse ion channel proteins, including voltage, stretch, and ligandactivated cation channels. Although tarantula toxins have been shown to partition into membranes, and the membrane is thought to play an important role in their activity, the structural interactions between these toxins and lipid membranes are poorly understood. Here, we use solid-state NMR and neutron diffraction to investigate the interactions between a voltage sensor toxin (VSTx1) and lipid membranes, with the goal of localizing the toxin in the membrane and determining its influence on membrane structure. Our results demonstrate that VSTx1 localizes to the headgroup region of lipid membranes and produces a thinning of the bilayer. The toxin orients such that many basic residues are in the aqueous phase, all three Trp residues adopt interfacial positions, and several hydrophobic residues are within the membrane interior. One remarkable feature of this preferred orientation is that the surface of the toxin that mediates binding to voltage sensors is ideally positioned within the lipid bilayer to favor complex formation between the toxin and the voltage sensor.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Vesicle interactions, Biochemistry