Title
Structure Change from β-Strand and Turn to α-Helix in Histone H2A-H2B Induced by DNA Damage Response
Author
Yudai Izumi, Kentaro Fujii, Frank Wien, Chantal Houée-Lévin, Sandrine Lacombe, Daniela Salado-Leza, Erika Porcel, Rawand Masoud, Satoshi Yamamoto, Matthieu Réfrégiers, Marie-Anne Hervé du Penhoat, Akinari Yokoya
Year
2016
Journal
Biophysical Journal
Abstract
Using synchrotron radiation-based circular dichroism spectroscopy, we found that the DNA damage response induces an increase of α-helix structure and a decrease of β-strand and turn structures in histone H2A-H2B extracted from x-irradiated human HeLa cells. The structural alterations correspond to the assumption that an average of eight amino acid residues form new α-helix structures at 310 K. We propose the structural transition from β-strand and turn structures to an α-helix structure in H2A-H2B as a novel, to our knowledge, process involved in the DNA damage response.
Instrument
J-725
Keywords
Circular dichroism, Secondary structure, Thermal stability, Biochemistry