Structure-dependent binding of hnRNP A1 to telomere RNA

By Xiao Liu, Takumi Ishizuka, Hong-Liang Bao, Kei Wada, Yuuma Takada, Keisuke Iida, Kazuo Nagasawa, Danzhou Yang, Yan Xu

July 28, 2017

  • Year

  • Technique

  • Industry

Title

Structure-dependent binding of hnRNP A1 to telomere RNA

Author

Xiao Liu, Takumi Ishizuka, Hong-Liang Bao, Kei Wada, Yuuma Takada, Keisuke Iida, Kazuo Nagasawa, Danzhou Yang, Yan Xu

Year

2017

Journal

JACS

Abstract

Telomeric repeat-containing RNA is a new non-coding RNA molecule that performs various bio-functions. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein involved in the telomere maintenance machinery. To date, little is known about how hnRNPA1 binds to telomeric RNA. In this study, we investigated the binding affinity and recognition mechanism of telomere RNA with the RNA recognition motif (RRM) of hnRNPA1. Using the photochemical crosslinking method, we showed that the telomere RNA G-quadruplex with loops is important in the interaction of telomere RNA with hnRNPA1. Using small molecule probes, we directly visualized the complex formed by the telomere RNA G-quadruplex and hnRNPA1 in vitro and in live cells. The results suggested that the structure-dependent binding of hnRNPA1 to telomere RNA regulates the telomere function. Therefore, our study provides new insights into the interactions between the RNA G-quadruplex and proteins at the telomere.

Full Article

http://pubs.acs.org/doi/abs/10.1021/jacs.7b01599

Instrument

J-820

Keywords

Circular dichroism, RNA structure, Ligand binding, Thermal stability, Thermodynamics, Biochemistry