Title
Study on the interaction between Fe3+ and fibrinogen and its influence on the polymerization behavior of fibrin networks
Author
Lei Wang, Rui Li, Lianzhi Li, Huaisheng Wang, Jifeng Liu
Year
2016
Journal
RSC Advances
Abstract
The interactions between fibrinogen molecules and Fe3+ were investigated with UV-Vis spectra, circular dichroism spectra, solution turbidity, steady viscosities, zeta potential, average particle size and size distribution and the molecular docking method. The polymerization behaviour of a fibrinogen solution mediated by different concentrations of Fe3+ was studied by atomic force microscopy. The results indicated that overloading Fe3+ in the fibrinogen solution will accelerate the amorphous aggregation of fibrin, leading to the formation of large rigid clot structures and an elevated fiber mass to length ratio.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Ligand binding, Biochemistry