Title
Sub-micron sized cytochrome c particles adsorbing to solid surfaces: A comparison between solution phase and colloidal system
Author
Maheshkumar Jaganathan, S. Easwaramoorthy, A. Dhathathreyan
Year
2019
Journal
International Journal of Biological Macromolecules
Abstract
Present work reports on Cytochrome C (Cyt C) adsorption from solution and as sub-micron sized colloidal particles (pH 7.5) at fluid/solid interface. The colloidal particles from 2 methods ((method 1 freeze-thaw); (method 2-Decane/water interface)) have been characterized by UV–Visible spectroscopy, Static and Time resolved Fluorescence spectra and Circular dichroic spectroscopy. Morphology and size studies indicate that the colloids are solid and well-packed. Using Quartz crystal microbalance with dissipation, elastic compliance of the protein on quartz surface has been monitored. Properties of the protein from solution and as colloids assembled from method 2 are similar in elastic compliance (65.53 ± 1.2 and 73.5 ± 1.1 GPa−1 respectively) due to polar/non-polar interactions at the solid surface. For particles from method 1, irregular desolvation of water on the particle surface results in higher compliance (104.3 ± 1.3 GPa−1). Change in work of adhesion from contact angle profiles shows optimal adhesion for colloids from method 1 whereas the protein solution and as colloids from method 2, show subnormal adhesion. The work shows the role of extensive H-bonding with the hydrodynamically coupled water leading to a fluid like system in protein colloids from method 1 whereas those from method 2 behaved more like the pure protein adsorbing from solution.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Solid state, Biochemistry