Title
Synthesis and characterization of manganese ferrite nanoparticles, and its interaction with bovine serum albumin: A spectroscopic and molecular docking approach
Author
Sona Gandhi, Indrajit Roy
Year
2019
Journal
Journal of Molecular Liquids
Abstract
Magnetic nanoparticles are attractive for biomedical applications as they can be used for magnetic targeting of drugs, as contrast agents for MRI, and as externally-activated therapeutic agents (e.g. in magnetic hyperthermia). The understanding of biological interactions of the magnetic particles is mandatory for their successful clinical use, as these interactions give insights into blood circulation and biodistribution of the nanoparticles. In this work, we have synthesized manganese ferrite nanoparticles and investigated their interactions with bovine serum albumin (BSA) using various spectroscopic techniques. The nanoparticles were synthesized facilely through precipitation and were characterized by TEM, SEM, XRD, VSM, and DLS. The particles quenched the fluorescence of BSA statically indicating complex formation, established by Stern-Volmer equation and time-resolved fluorescence measurements. The binding constant is determined using UV–visible absorption and fluorescence spectroscopies. The structural integrity of BSA is not lost upon binding, as seen by circular dichroism data, indicating biocompatibility and potential for biomedical use. Molecular docking studies were used to identify the main amino acid residues within BSA involved in the interaction with the nanoparticles.
Instrument
J-810
Keywords
Circular dichroism, Ligand binding, Nanostructures, Secondary structure, Biochemistry, Materials