Synthesis of triangular silver nanoprisms and studies on the interactions with human serum albumin
Xiangyu Xu, Yunfei Wang, Hao Wang, Huaifen Su, Xuyan Mao, Liang Jiang, Min Liu, Dezhi Sun, Shifeng Hou
Journal of Molecular Liquids
Biofunctionalization of noble metal nanoparticles (such as Ag) is necessary to obtain biocompatibility for specific biomedical applications. Silver nanoprisms (AgNPrs) are being increasingly used owing to their outstanding optoelectronic properties. This study aims to investigate the interaction between triangular silver nanoprisms (AgNPrs) and human serum albumin (HSA) in aqueous solution. Analysis of the quenching data using Stern-Volmer method was used to reveal whether the HSA-AgNPrs complex could be formed and if the static quenching could happen with the interaction. Thermodynamic parameters, i.e., binding equilibrium constants (Kθ), standard changes of enthalpy (ΔHθ), Gibbs free energy (ΔGθ) and entropy (ΔSθ) for the binding process at different temperatures have been derived from the fluorescence spectra. These parameters were utilized to reveal the thermodynamic driving force of the interaction process. Furthermore, what kind of role the hydrogen bonding or electrostatic interaction might play in the interacting process was judged based on the fluorescence measurements. The data and message of UV–visible, circular dichroism, and Fourier transform infrared (FT-IR) spectra suggested that the secondary structure of HSA undergoes a decrease in α-helix with the increasing relative amount of AgNPrs in the aqueous system.
Circular dichroism, Secondary structure, Ligand binding, Nanostructures, Biochemistry, Materials