Title
Synthesis, Spectral Properties and DFT Calculations of new Ruthenium (II) Polypyridyl Complexes; DNA Binding Affinity and in Vitro Cytotoxicity Activity
Author
Rajender Reddy Mallepally, Nagamani Chintakuntla, Venkat Reddy Putta, Nagasuryaprasad K, Ravi Kumar Vuradi, Madhuri P Satyanarayana Singh S, Ramesh Kumar Chitumalla, Joonkyung Jang, Nagababu Penumaka, Satyanarayana Sirasani
Year
2017
Journal
Journal of Fluorescence
Abstract
In this paper a novel ligand debip (2–(4–N,N–diethylbenzenamine)1H–imidazo[4,5–f] [1, 10]phenanthroline) and its Ru(II) polypyridyl complexes [Ru(L)2(debip)]2+, (L = phen (1), bpy (2) and dmb (3)) have been synthesized and characterized by spectroscopic techniques. The DNA binding studies for all these complexes were examined by absorption, emission, quenching studies, viscosity measurements and cyclic voltammetry. The light switching properties of complexes 1–3 have been evaluated. Molecular docking, Density Functional Theory (DFT) and time dependent DFT calculations were performed. The Ru(II) complexes exhibited efficient photocleavage activity against pBR322 DNA upon irradiation and exhibited good antimicrobial activity. Also investigated 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, lactate dehydrogenase (LDH) release assay and reactive oxygen species (ROS) against selected cancer cell lines (HeLa, PC3, Lancap, MCF-7 and MD-MBA 231).
Instrument
FP-8500
Keywords
Fluorecence, DNA binding, Inorganic chemistry, Materials