The 5′-AG₅CC-3′ fragment from the human CPEB3 ribozyme forms an ultrastable parallel RNA G-quadruplex

By Katharina Kulikov, Senada Nozinovic, Stephanie Kath-Schorr

July 28, 2017

  • Year

  • Technique

  • Industry

Title

The 5′-AG₅CC-3′ fragment from the human CPEB3 ribozyme forms an ultrastable parallel RNA G-quadruplex

Author

Katharina Kulikov, Senada Nozinovic, Stephanie Kath-Schorr

Year

2017

Journal

ChemBioChem

Abstract

An unusually thermostable G-quadruplex is formed by a sequence fragment of a naturally occurring ribozyme, the human CPEB3 ribozyme. Strong evidence is provided for the formation of a uniquely stable intermolecular G-quadruplex structure consisting of five tetrad layers using CD-spectroscopy, UV-melting curves, two-dimensional NMR spectroscopy and gel shift analysis. The cationic porphyrin TMPyP4 destabilizes the complex.

Full Article

http://onlinelibrary.wiley.com/wol1/doi/10.1002/cbic.201700028/abstract

Instrument

J-810

Keywords

Circular dichroism, DNA structure, Ligand binding, Biochemistry