Title
The reaction of dichlorodiammineplatinum(II), [PtCl2(NH3)2], isomers with zinc fingers
Author
Samantha D. Tsotsoros, Yun Qu, Nicholas P. Farrell
Year
2015
Journal
Journal of Inorganic Biochemistry
Abstract
The interaction of cis-DDP and trans-DDP (DDP = [PtCl2(NH3)2]) with the C-terminal zinc finger (ZF2) of the HIVNCp7 nucleocapsid protein was investigated by fluorescence, circular dichroism, mass spectrometry, and {1H, 15N} HSQC (heteronuclear single quantum coherence) NMR spectroscopy. The rate of reaction differed significantly for the two compounds, with the trans isomer reacting in a significantly faster manner, as expected. {1H, 15N} HSQC NMR of 15N-labeled compounds with the ZF2 showed the appearance of several new 15N peaks, consistent with sulfur binding and formation of Pt-S species. Mass spectrometry confirmed the formation of several different Pt-apopeptide/ZF2 adducts. Circular dichroism and fluorescence spectroscopy also indicated conformational changes upon binding while a 33% decrease in fluorescence of the unique tryptophan residue was seen in 72 h upon complexation of the cis isomer, while the trans isomer quenched 50% in just 24 h.
Instrument
J-1500
Keywords
Circular dichroism, Inorganic chemistry, Ligand binding, Biochemistry